Cardiac differentiation of pluripotent stem cells

2020-02-29 12:44 Summary. Human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells, are potentially useful in regenerative therapies for heart disease. For medical applications, clinicalgrade cardiac cells must be produced from hPSCs in a

Apr 04, 2018  DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CM SUBTYPES. Cardiac mesoderm was formed from hPSCs using a low concentration of Activin A and BMP4, and then was treated with a low concentration of BMP4 and a high concentration of retinoic acid to generate functional sinoatrial nodelike cells ( 47 ). cardiac differentiation of pluripotent stem cells

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An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures. Stem Cell Res. 13, (2014). cardiac differentiation of pluripotent stem cells

The first step (i. e. , mesoderm induction) in cardiac differentiation from pluripotent stem cells has been well characterized. Numerous studies have demonstrated that Wnts, BMPs, and transforming growth factor (TGF) family member Nodal (or Activin A as a substitute of Nodal) efficiently induce mesoderm [50, 51. Substratedependent cardiac differentiation of induced pluripotent stem cells (iPSCs) has been studied on various extracellular matrix (ECM)derived substrates, such as collagen type I (ColI). Furthermore, the cardiac differentiation efficacy of a given protocol is different when applied to different pluripotent stem cell lines. Sometimes, a given protocol and cell line can even yield significant differences from one experiment to the next in one laboratory. cardiac differentiation of pluripotent stem cells Here, using direct reprogrammingbased screening, singlecell RNAseq in mouse embryos, and directed cardiac differentiation in pluripotent stem cells (PSCs), we demonstrated that Tbx6 induces nascent mesoderm from PSCs and determines cardiovascular and somite lineage specification via its temporal expression. Cardiac differentiation of pluripotent stem cells and implications for modeling the heart in health and disease Harsha D. Devalla, 1 and Robert Passier1, 2 Cellular models comprising cardiac cell types derived from human pluripotent stem cells are valuable for studying heart development and disease. The ability of human pluripotent stem cells to differentiate towards the cardiac lineage has attracted significant interest, initially with a strong focus on regenerative medicine. Abstract. Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a

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